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permeable inserts  (CELLTREAT Scientific)


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    Structured Review

    CELLTREAT Scientific permeable inserts
    Permeable Inserts, supplied by CELLTREAT Scientific, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+inserts/Permeable+Cell+Culture+Inserts/pmc13120886-22-11-18
    Average 94 stars, based on 6 article reviews
    permeable inserts - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Extracellular vesicles regulate metastable phenotypes of lymphangioleiomyomatosis cells via shuttling ATP synthesis to pseudopodia and activation of integrin adhesion complexes
    Article Snippet: .. In brief, cell culture inserts with 3.0 μm-pore polycarbonate membranes (CELLTREAT, #230609) were coated with collagen at a concentration of 50 μg/cm 2 for 2 hours at room temperature, rinsed with PBS, and seeded with cells from dissociated spheres. ..

    Article Title: Tumor-Associated Macrophages Expand Chemoresistant, Ovarian Cancer Stem-Like Cells
    Article Snippet: .. M2 macrophages were polarized on the upper surface of 0.4-μm cell culture inserts (Corning and CELLTREAT). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. S. mutans colony biofilms were cultured on a 1% Mueller–Hinton (MH) agar plate with different supplements by placing a droplet (3 μL) of S. mutans culture in the mid-exponential phase optical density (OD) 600 = 0.5 (3 × 10 8 colony-forming units, CFU/mL) on the cell culture inserts with polyethylene terephthalate (PET) membrane (0.4 μm pore size, PET membrane, sterile, 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) on the agar surface. ..

    Article Title: Extracellular vesicles modulate integrin signaling and subcellular energetics to promote pulmonary lymphangioleiomyomatosis metastasis.
    Article Snippet: .. In brief, cell culture inserts with 3.0 μm-pore polycarbonate membranes (CELLTREAT, #230609) were coated with collagen at a concentration of 50 μg/cm2 for 2 h at room temperature, rinsed with PBS, and seeded with cells from dissociated spheres. ..

    Article Title: Extracellular vesicles modulate integrin signaling and subcellular energetics to promote pulmonary lymphangioleiomyomatosis metastasis
    Article Snippet: .. In brief, cell culture inserts with 3.0 μm-pore polycarbonate membranes (CELLTREAT, #230609) were coated with collagen at a concentration of 50 μg/cm 2 for 2 h at room temperature, rinsed with PBS, and seeded with cells from dissociated spheres. ..

    Article Title: Influence of Insert Brand and Culture Method on Ciliary Activity and Epithelial Cell Types in Human Nasal Air–Liquid Interface Cell Cultures
    Article Snippet: .. Recently, Brocke et al. [ ] compared two commercially available cell culture inserts from CELLTREAT ® and Corning ® Transwell ® , finding no statistically significant differences in the cell differentiation outcomes of the resulting hNECs grown at the ALI. .. In this study, we conducted a comparative analysis of primary hNEC cultures from nineteen healthy non-smoking adult donors, cultivated at the ALI using three distinct commercially available culture inserts: Corning ® Transwell ® , CELLTREAT ® , and ThinCert ® .

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Concentration Assay:

    Article Title: Extracellular vesicles regulate metastable phenotypes of lymphangioleiomyomatosis cells via shuttling ATP synthesis to pseudopodia and activation of integrin adhesion complexes
    Article Snippet: .. In brief, cell culture inserts with 3.0 μm-pore polycarbonate membranes (CELLTREAT, #230609) were coated with collagen at a concentration of 50 μg/cm 2 for 2 hours at room temperature, rinsed with PBS, and seeded with cells from dissociated spheres. ..

    Article Title: Extracellular vesicles modulate integrin signaling and subcellular energetics to promote pulmonary lymphangioleiomyomatosis metastasis.
    Article Snippet: .. In brief, cell culture inserts with 3.0 μm-pore polycarbonate membranes (CELLTREAT, #230609) were coated with collagen at a concentration of 50 μg/cm2 for 2 h at room temperature, rinsed with PBS, and seeded with cells from dissociated spheres. ..

    Article Title: Extracellular vesicles modulate integrin signaling and subcellular energetics to promote pulmonary lymphangioleiomyomatosis metastasis
    Article Snippet: .. In brief, cell culture inserts with 3.0 μm-pore polycarbonate membranes (CELLTREAT, #230609) were coated with collagen at a concentration of 50 μg/cm 2 for 2 h at room temperature, rinsed with PBS, and seeded with cells from dissociated spheres. ..

    Suspension:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Sterility:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. S. mutans colony biofilms were cultured on a 1% Mueller–Hinton (MH) agar plate with different supplements by placing a droplet (3 μL) of S. mutans culture in the mid-exponential phase optical density (OD) 600 = 0.5 (3 × 10 8 colony-forming units, CFU/mL) on the cell culture inserts with polyethylene terephthalate (PET) membrane (0.4 μm pore size, PET membrane, sterile, 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) on the agar surface. ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Membrane:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. S. mutans colony biofilms were cultured on a 1% Mueller–Hinton (MH) agar plate with different supplements by placing a droplet (3 μL) of S. mutans culture in the mid-exponential phase optical density (OD) 600 = 0.5 (3 × 10 8 colony-forming units, CFU/mL) on the cell culture inserts with polyethylene terephthalate (PET) membrane (0.4 μm pore size, PET membrane, sterile, 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) on the agar surface. ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Pore Size:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. S. mutans colony biofilms were cultured on a 1% Mueller–Hinton (MH) agar plate with different supplements by placing a droplet (3 μL) of S. mutans culture in the mid-exponential phase optical density (OD) 600 = 0.5 (3 × 10 8 colony-forming units, CFU/mL) on the cell culture inserts with polyethylene terephthalate (PET) membrane (0.4 μm pore size, PET membrane, sterile, 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) on the agar surface. ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Positron Emission Tomography:

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries.
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. S. mutans colony biofilms were cultured on a 1% Mueller–Hinton (MH) agar plate with different supplements by placing a droplet (3 μL) of S. mutans culture in the mid-exponential phase optical density (OD) 600 = 0.5 (3 × 10 8 colony-forming units, CFU/mL) on the cell culture inserts with polyethylene terephthalate (PET) membrane (0.4 μm pore size, PET membrane, sterile, 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA) on the agar surface. ..

    Article Title: Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries
    Article Snippet: .. A 3 μL aliquot of this bacterial suspension was carefully pipetted onto a sterile filter membrane of cell culture inserts (0.4 μm pore size, PET membrane, sterile, packed in 12-well plate format; Celltreat Scientific Products, Pepperell, MA, USA). ..

    Cell Differentiation:

    Article Title: Influence of Insert Brand and Culture Method on Ciliary Activity and Epithelial Cell Types in Human Nasal Air–Liquid Interface Cell Cultures
    Article Snippet: .. Recently, Brocke et al. [ ] compared two commercially available cell culture inserts from CELLTREAT ® and Corning ® Transwell ® , finding no statistically significant differences in the cell differentiation outcomes of the resulting hNECs grown at the ALI. .. In this study, we conducted a comparative analysis of primary hNEC cultures from nineteen healthy non-smoking adult donors, cultivated at the ALI using three distinct commercially available culture inserts: Corning ® Transwell ® , CELLTREAT ® , and ThinCert ® .



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    Image Search Results


    Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding onto temperature-responsive culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.

    Journal: Regenerative Therapy

    Article Title: Characterization of the role and fate of nasal mucosal epithelial cell sheets in middle ear regeneration

    doi: 10.1016/j.reth.2026.101130

    Figure Lengend Snippet: Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding onto temperature-responsive culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.

    Article Snippet: The cells were then seeded onto temperature-responsive cell culture inserts (CellSeed) at a density of 4 × 10 4 cells/cm 2 and cultured in KCM for 7 days.

    Techniques: Derivative Assay, Incubation, Fluorescence, Microscopy, Immunohistochemical staining, Staining